| School | Location | Conf. | Head Coach |
|---|
| Adrian Bulldogs | Adrian, MI | MIAA | Evan Himes (Hiram '03) |
| Albion Britons | Albion, MI | MIAA | Jerry Block (DePauw '95) |
| Alma Scots | Alma, MI | MIAA | Nathan Kronewetter (Hope '96) |
| Augustana Vikings | Rock Island, IL | CCIW | Brenton Joseph (Alabama A&M '93) |
| Aurora Spartans | Aurora, IL | NATHC | Pete Watkins (MacMurray) |
| Benedictine Eagles | Lisle, IL | NATHC | Rob Kettle (Warwick University '83) |
| Blackburn Beavers | Carlinville, IL | SLIAC | Aydin Gonulsen (Warren Wilson) |
| Calvin Knights | Grand Rapids, MI | MIAA | Chris Hughes (Calvin '92) |
| Carthage Red Men | Kenosha, WI | CCIW | Steve Domin (Carthage '93) |
| Chicago Maroons | Chicago, IL | UAA | Scott Wiercinski (Middlebury '90) |
| Concordia (Ill.) Cougars | River Forest, IL | NATHC | Micah Middendorf (Concordia, Wis. '05) |
| Concordia (Wis.) Falcons | Mequon, WI | NATHC | Tom Saleska |
| Dominican Stars | River Forest, IL | NATHC | Erick Baumann (Dominican '93) |
| Edgewood Eagles | Madison, WI | NATHC | Tim Alexander (UW-Green Bay) |
| Elmhurst Bluejays | Elmhurst, IL | CCIW | Dave Di Tomasso (Jacksonville) |
| Eureka Red Devils | Eureka, IL | SLIAC | Randy Crady (Bradley) |
| Fontbonne Griffins | St. Louis, MO | SLIAC | Brian Hoener (Fontbonne) |
| Greenville Panthers | Greenville, IL | SLIAC | Brian Mcmahon (Indiana Wesleyan '98) |
| Hendrix Warriors | Conway, AR | SCAC | Doug Mello |
| Hope Flying Dutchmen | Holland, MI | MIAA | Steve Smith (Grand Rapids Baptist '81) |
| Illinois Wesleyan Titans | Bloomington, IL | CCIW | Ryan Lakin (Illinois State '95) |
| Kalamazoo Hornets | Kalamazoo, MI | MIAA | Chris Adrian (Drake '99) |
| Lakeland Muskies | Sheboygan, WI | NATHC | David Madsend (UW-Whitewater '03) |
| MacMurray Highlanders | Jacksonville, IL | SLIAC | Randy Hall (Lincoln University) |
| Maranatha Baptist Crusaders | Watertown, WI | NATHC | Jeff Pill |
| Marian Sabres | Fond du Lac, WI | NATHC | Sean Bray (IUPUI '95) |
| Millikin Big Blue | Decatur, IL | CCIW | B.J. Noble (Northern State '96) |
| North Central (Ill.) Cardinals | Naperville, IL | CCIW | Matt Klosterman (Loras '99) |
| North Park Vikings | Chicago, IL | CCIW | John Born (Concordia, Wis.) |
| Olivet Comets | Olivet, MI | MIAA | Bill Boyle (Hartwick '98) |
| Principia Panthers | Elsah, IL | SLIAC | Vitalis Otieno (Principia) |
| Rust Bearcats | Holly Springs, MS | IND | Abdoul Yessoufou |
| Spalding Golden Eagles | Louisville, KY | SLIAC | Kevin Cromwell (Missouri Valley '09) |
| Trine Thunder | Angola, IN | MIAA | Mike Ferrell (Campbell '79) |
| University of Dallas Crusaders | Dallas, TX | NEAC | David Hoffman (St. Mary's, Texas) |
| Washington U. Bears | Saint Louis, MO | UAA | Joe Clarke (St. Louis University '76) |
| Webster Gorloks | St. Louis, MO | SLIAC | Marty Todt |
| Westminster (Mo.) Blue Jays | Fulton, MO | SLIAC | Eric Steege (St. Olaf '04) |
| Wheaton (Ill.) Thunder | Wheaton, IL | CCIW | Michael Giuliano (Tennessee Temple '81) |
| Wisconsin Lutheran Warriors | Milwaukee, WI | NATHC | Joe Luedke (UW-Milwaukee) |
